The study of biological enzymes with their unique properties such as substrate interactions, conformational changes, and catalytic rates is a key to basic and applied enzymology research. Among enzymes, horseradish peroxidase (HRP) has been widely used in studying the metabolism of biological systems. In this study, we measure changes in the activity and structure of horseradish peroxidase in both laboratory grade and isoforms extracted from the plant under the influence of the Faradarmani Consciousness Field (FCF). Our objectives were to first test the effect of the FCF on the structure and function of HRP, and secondly, analyze whether or not changes in the maximum velocity and Michaelis constant were reproducible under the influence of FCF. We observed that the laboratory grade HRP had a 5% decrease in maximum velocity and 18% increase in Michaelis constant as a result of FCF treatment when compared to the non-treated controls. On the other hand, the HRP extracted from the plant tissue showed a 6% and 40% increase in maximum velocity and Michaelis constant, respectively, under the FCF. Additionally, changes in the fluorescence emission intensity spectrum suggest both structural and functional changes in this enzyme as a result of the FCF treatment.
Keywords: Faradarmani Consciousness Fields (FCF); Michaelis constant; maximum velocity; horseradish peroxidase